The Railway Museum of Athens, Greece, was founded by the Hellenic Railways Organisation (OSE) in 1978. It is currently located at 4 Siokou Street, next to the mainline from Athens to Inoi. The museum has a collection of items related to the history of rail transport in Greece, many of them exhibited in its five rooms. The museum is open daily from 09:00 to 13:00 except Mondays, and is also open on Wednesday evenings from 17:00 to 20:00. Admission is free. Photography in the museum is permitted from 2015 onwards (was prohibited in the past).

The museum has very limited space. The rolling stock collection is exhibited in Hall B and consists mainly of 1,000 mm (3 ft 3 ³⁄₈ in) metre gauge vehicles. Exhibited rolling stock includes the following:

– Steam lecomotives dating from 1884,

– Steam locomotives of mine-trains,

– Wagons of old trains, royal riages and the smoking car of the train of the sultan Abdul Aziz,

– Athenian tramways of the past decades,

– Hand-and-foot operated draeseners,

– Models and photographic material,

– Instruments, printing implements, tickets, uniforms and mechanics’ tools of the 19th century.